eif 2α Search Results


sal003  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sal003
Figure 3. Phosphorylation of eIF2a during glucose deprivation. Western blots of extracts from HepG2 cells incubated for 6 hr in media having either 0 mM or 5 mM glucose, and without (-) or with (+) 10 mM <t>Sal003</t> as shown above lanes. Antibodies against phospho-eIF2a (eIF2a,P), total eIF2a and GAPDH as a loading control, were used as indicated to the right of each blot. doi:10.1371/journal.pone.0040994.g003 Figure 4. Glucose concentration affects secreted FVII antigen levels. HepG2 cells were cultured for 24 hr in media supplemented with 10% or 1% fetal bovine serum and either high (25 mM), standard (5 mM), or low/no (1 mM or 0 mM) glucose. The concentration of secreted FVII antigen, expressed as ng per ml, was determined by ELISA. For each experimental set, the average amount secreted by cells in 25 mM glucose was considered 100%, and amounts secreted at the lower glucose concentrations were expressed as percentages +/2 SD relative to that. The number of replicates assayed at each condition is shown below the bars. Reducing the concentration of glucose significantly increased the amount of FVII secreted for each experi- mental set (p,0.001). doi:10.1371/journal.pone.0040994.g004
Sal003, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sal003/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
sal003 - by Bioz Stars, 2026-03
93/100 stars
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phospho-eif-2α (ser51) rabbit polyclonal antibody  (Xiamen Biotime Biotechnology Co Ltd)
90
Xiamen Biotime Biotechnology Co Ltd phospho-eif-2α (ser51) rabbit polyclonal antibody
Figure 3. Phosphorylation of eIF2a during glucose deprivation. Western blots of extracts from HepG2 cells incubated for 6 hr in media having either 0 mM or 5 mM glucose, and without (-) or with (+) 10 mM <t>Sal003</t> as shown above lanes. Antibodies against phospho-eIF2a (eIF2a,P), total eIF2a and GAPDH as a loading control, were used as indicated to the right of each blot. doi:10.1371/journal.pone.0040994.g003 Figure 4. Glucose concentration affects secreted FVII antigen levels. HepG2 cells were cultured for 24 hr in media supplemented with 10% or 1% fetal bovine serum and either high (25 mM), standard (5 mM), or low/no (1 mM or 0 mM) glucose. The concentration of secreted FVII antigen, expressed as ng per ml, was determined by ELISA. For each experimental set, the average amount secreted by cells in 25 mM glucose was considered 100%, and amounts secreted at the lower glucose concentrations were expressed as percentages +/2 SD relative to that. The number of replicates assayed at each condition is shown below the bars. Reducing the concentration of glucose significantly increased the amount of FVII secreted for each experi- mental set (p,0.001). doi:10.1371/journal.pone.0040994.g004
Phospho Eif 2α (Ser51) Rabbit Polyclonal Antibody, supplied by Xiamen Biotime Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho-eif-2α (ser51) rabbit polyclonal antibody/product/Xiamen Biotime Biotechnology Co Ltd
Average 90 stars, based on 1 article reviews
phospho-eif-2α (ser51) rabbit polyclonal antibody - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti-eif-2α  (Servicebio Inc)
90
Servicebio Inc rabbit anti-eif-2α
Quinoa may regulate TGR5, GLP-1, TLR4 and endoplasmic reticulum (ER) stress in the liver. (A) IHC of phosphorylated eukaryotic initiation factor 2 alpha <t>(eIF-2α)</t> in the liver. (B) IHC of TGR5 in the liver. (C) IHC of GLP-1 in the liver. (D) IHC of TLR4 in the liver. (E) Quantification AOD values of eIF-2α and TGR5 in the liver by Image J. (F) Quantification AOD values of GLP-1 and TLR4 in the liver by Image J. (G) Representative Western blot of the transcription factor C/EBP homologous protein (CHOP) and eIF-2α in the liver. (H) Quantitative assessment of the Western blot analysis results of CHOP and eIF-2α by Image J. *, P <0.05; **, P <0.01; compared with the control group and #, P <0.05; ##, P <0.01, versus the HFD group, determined by ANOVA and non-parametric test.
Rabbit Anti Eif 2α, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-eif-2α/product/Servicebio Inc
Average 90 stars, based on 1 article reviews
rabbit anti-eif-2α - by Bioz Stars, 2026-03
90/100 stars
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salubrinal  (Merck & Co)
90
Merck & Co salubrinal
Quinoa may regulate TGR5, GLP-1, TLR4 and endoplasmic reticulum (ER) stress in the liver. (A) IHC of phosphorylated eukaryotic initiation factor 2 alpha <t>(eIF-2α)</t> in the liver. (B) IHC of TGR5 in the liver. (C) IHC of GLP-1 in the liver. (D) IHC of TLR4 in the liver. (E) Quantification AOD values of eIF-2α and TGR5 in the liver by Image J. (F) Quantification AOD values of GLP-1 and TLR4 in the liver by Image J. (G) Representative Western blot of the transcription factor C/EBP homologous protein (CHOP) and eIF-2α in the liver. (H) Quantitative assessment of the Western blot analysis results of CHOP and eIF-2α by Image J. *, P <0.05; **, P <0.01; compared with the control group and #, P <0.05; ##, P <0.01, versus the HFD group, determined by ANOVA and non-parametric test.
Salubrinal, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/salubrinal/product/Merck & Co
Average 90 stars, based on 1 article reviews
salubrinal - by Bioz Stars, 2026-03
90/100 stars
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eif-2α kinase # 2002  (Portland Press Ltd)
90
Portland Press Ltd eif-2α kinase # 2002
Quinoa may regulate TGR5, GLP-1, TLR4 and endoplasmic reticulum (ER) stress in the liver. (A) IHC of phosphorylated eukaryotic initiation factor 2 alpha <t>(eIF-2α)</t> in the liver. (B) IHC of TGR5 in the liver. (C) IHC of GLP-1 in the liver. (D) IHC of TLR4 in the liver. (E) Quantification AOD values of eIF-2α and TGR5 in the liver by Image J. (F) Quantification AOD values of GLP-1 and TLR4 in the liver by Image J. (G) Representative Western blot of the transcription factor C/EBP homologous protein (CHOP) and eIF-2α in the liver. (H) Quantitative assessment of the Western blot analysis results of CHOP and eIF-2α by Image J. *, P <0.05; **, P <0.01; compared with the control group and #, P <0.05; ##, P <0.01, versus the HFD group, determined by ANOVA and non-parametric test.
Eif 2α Kinase # 2002, supplied by Portland Press Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eif-2α kinase # 2002/product/Portland Press Ltd
Average 90 stars, based on 1 article reviews
eif-2α kinase # 2002 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Figure 3. Phosphorylation of eIF2a during glucose deprivation. Western blots of extracts from HepG2 cells incubated for 6 hr in media having either 0 mM or 5 mM glucose, and without (-) or with (+) 10 mM Sal003 as shown above lanes. Antibodies against phospho-eIF2a (eIF2a,P), total eIF2a and GAPDH as a loading control, were used as indicated to the right of each blot. doi:10.1371/journal.pone.0040994.g003 Figure 4. Glucose concentration affects secreted FVII antigen levels. HepG2 cells were cultured for 24 hr in media supplemented with 10% or 1% fetal bovine serum and either high (25 mM), standard (5 mM), or low/no (1 mM or 0 mM) glucose. The concentration of secreted FVII antigen, expressed as ng per ml, was determined by ELISA. For each experimental set, the average amount secreted by cells in 25 mM glucose was considered 100%, and amounts secreted at the lower glucose concentrations were expressed as percentages +/2 SD relative to that. The number of replicates assayed at each condition is shown below the bars. Reducing the concentration of glucose significantly increased the amount of FVII secreted for each experi- mental set (p,0.001). doi:10.1371/journal.pone.0040994.g004

Journal: PloS one

Article Title: Upregulation of the coagulation factor VII gene during glucose deprivation is mediated by activating transcription factor 4.

doi: 10.1371/journal.pone.0040994

Figure Lengend Snippet: Figure 3. Phosphorylation of eIF2a during glucose deprivation. Western blots of extracts from HepG2 cells incubated for 6 hr in media having either 0 mM or 5 mM glucose, and without (-) or with (+) 10 mM Sal003 as shown above lanes. Antibodies against phospho-eIF2a (eIF2a,P), total eIF2a and GAPDH as a loading control, were used as indicated to the right of each blot. doi:10.1371/journal.pone.0040994.g003 Figure 4. Glucose concentration affects secreted FVII antigen levels. HepG2 cells were cultured for 24 hr in media supplemented with 10% or 1% fetal bovine serum and either high (25 mM), standard (5 mM), or low/no (1 mM or 0 mM) glucose. The concentration of secreted FVII antigen, expressed as ng per ml, was determined by ELISA. For each experimental set, the average amount secreted by cells in 25 mM glucose was considered 100%, and amounts secreted at the lower glucose concentrations were expressed as percentages +/2 SD relative to that. The number of replicates assayed at each condition is shown below the bars. Reducing the concentration of glucose significantly increased the amount of FVII secreted for each experi- mental set (p,0.001). doi:10.1371/journal.pone.0040994.g004

Article Snippet: Thapsigargin (Calbiochem EMD Millipore, Billerica, MA) at 500 nM final and Sal003 (Santa Cruz Biotechnology, Santa Cruz, CA) at 10 mM final were added to cultures for 6 hr where indicated.

Techniques: Phospho-proteomics, Western Blot, Incubation, Control, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

Quinoa may regulate TGR5, GLP-1, TLR4 and endoplasmic reticulum (ER) stress in the liver. (A) IHC of phosphorylated eukaryotic initiation factor 2 alpha (eIF-2α) in the liver. (B) IHC of TGR5 in the liver. (C) IHC of GLP-1 in the liver. (D) IHC of TLR4 in the liver. (E) Quantification AOD values of eIF-2α and TGR5 in the liver by Image J. (F) Quantification AOD values of GLP-1 and TLR4 in the liver by Image J. (G) Representative Western blot of the transcription factor C/EBP homologous protein (CHOP) and eIF-2α in the liver. (H) Quantitative assessment of the Western blot analysis results of CHOP and eIF-2α by Image J. *, P <0.05; **, P <0.01; compared with the control group and #, P <0.05; ##, P <0.01, versus the HFD group, determined by ANOVA and non-parametric test.

Journal: Microbiology Spectrum

Article Title: Quinoa Reduces High-Fat Diet-Induced Obesity in Mice via Potential Microbiota-Gut-Brain-Liver Interaction Mechanisms

doi: 10.1128/spectrum.00329-22

Figure Lengend Snippet: Quinoa may regulate TGR5, GLP-1, TLR4 and endoplasmic reticulum (ER) stress in the liver. (A) IHC of phosphorylated eukaryotic initiation factor 2 alpha (eIF-2α) in the liver. (B) IHC of TGR5 in the liver. (C) IHC of GLP-1 in the liver. (D) IHC of TLR4 in the liver. (E) Quantification AOD values of eIF-2α and TGR5 in the liver by Image J. (F) Quantification AOD values of GLP-1 and TLR4 in the liver by Image J. (G) Representative Western blot of the transcription factor C/EBP homologous protein (CHOP) and eIF-2α in the liver. (H) Quantitative assessment of the Western blot analysis results of CHOP and eIF-2α by Image J. *, P <0.05; **, P <0.01; compared with the control group and #, P <0.05; ##, P <0.01, versus the HFD group, determined by ANOVA and non-parametric test.

Article Snippet: IHC for GLP-1, claudin-1 in colons and brains as well as eIF-2α in livers were detected using the rabbit anti-GLP-1 (1:500; Servicebio), mouse anti-claudin-1 (1:500; Servicebio) and rabbit anti-eIF-2α (1:200; Servicebio) monoclonal antibodies.

Techniques: Western Blot